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Am. J. Trop. Med. Hyg., 29(6), 1980, pp. 1254-1257
Copyright © 1980 by The American Society of Tropical Medicine and Hygiene

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Immunodiagnosis of Infection with Schistosoma Haematobium and S. Mansoni in Man

George V. Hillyer, Reda M. R. Ramzy, M. A. El Alamy AND Barnett L. Cline
Laboratory of Parasite Immunology, Department of Biology, University of Puerto Rico, Río Piedras, Puerto Rico 00931 Center for Field and Applied Research, P.O. Box 38, Imbaba-Giza, Arab Republic of Egypt, and Department of Tropical Medicine, Tulane University School of Public Health and Tropical Medicine, New Orleans, Louisiana 70112

We utilized the circumoval precipitin (COP) test, Ouchterlony immunodiffusion, and the enzyme-linked immunosorbent assay (ELISA) for the serodiagnosis of human infection with Schistosoma mansoni or S. haematobium, or with both species of schistosome. Only the COP test correctly identified all of those with schistosome infection, although differentiation as to schistosome species was impossible. Circumoval precipitates around S. haematobium eggs from human urine were more numerous and larger than those around S. mansoni eggs obtained from mouse livers. Ouchterlony immunodiffusion with S. mansoni or S. haematobium worm extract failed to diagnose correctly approximately one of every five infected individuals. Mean absorption values of the S. mansoni soluble egg antigen (SEA) used in the ELISA were similar in serum samples from persons infected with either or both species of blood flukes. This suggests that S. mansoni SEA is not species-specific. The ELISA correctly identified, however, 30 of 32 infected individuals, indicating high sensitivity.

Accepted for publication April 26, 1980.






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Copyright © 1980 by the American Society of Tropical Medicine and Hygiene.