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Am. J. Trop. Med. Hyg., 29(6), 1980, pp. 1147-1149
Copyright © 1980 by The American Society of Tropical Medicine and Hygiene

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Separation of Stages of Plasmodium Falciparum-Infected Cells by means of a Fluorescence-Activated Cell Sorter

Graham V. Brown*, Francis L. Battye AND Russell J. Howard{dagger}
The Walter and Eliza Hall Institute of Medical Research, Post Office, Royal Melbourne Hospital, Victoria 3050, Australia

Plasmodium falciparum parasites from long-term in vitro culture have been labeled with the DNA-binding dye Hoechst 33258. After labeling, parasitized cells have been successfully analyzed and sorted, using a fluorescence-activated cell sorter, into populations of uninfected, singly infected, and multiply infected cells.

Accepted for publication June 14, 1980.


* To whom correspondence should be sent.


{dagger} Present address: Malaria Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20205.






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Copyright © 1980 by the American Society of Tropical Medicine and Hygiene.