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Recovery of Large Numbers of Eosinophils from Mice Infected with Toxocara canis^*

As a method of recovering large numbers of eosinophils, mice were infected with Toxocara canis which in 12 days produced a high peripheral eosinophilia; the mice were then injected intraperitoneally with an extract of T. canis adult or Anisakis larval worms. Two days after injection of extract, peritoneal exudates were withdrawn, washed, and enumerated; the exudates were observed to be 70–80% eosinophils with a mean total recovery of 1 x 10⁸ eosinophils per mouse.

Accepted for publication February 18, 1980.

^* Address reprint requests to: Kazuo Sugane, M. D., Department of Parasitology, School of Medicine, Yokohama City University, 2–33, Urafune-cho, Minamiku, Yokhoama 233, Japan