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Studies on Human Filariasis in Malaysia: The Application of an Indirect Hemagglutination Technioue for Immunodiagnosis^*

The indirect hemagglutination (IHA) test done with turkey red cells was applied to 173 serum samples obtained from patients and persons exposed to Wuchereria bancrofti and Brugia malayi in endemic areas of Peninsular Malaysia. A crude extract of adult worms of the rat filaria, Breinlia booliati, was used as the antigen. When a titer of 1:16 was taken as negative, positive IHA test rates in sera from microfilaria-negative persons in endemic areas, microfilaremic cases, and patients with clinical filariasis were 13%, 75%, and 80%, respectively. Results of the IHA test correlated well with results obtained with the indirect fluorescent technique.

Accepted for publication May 12, 1979.

^* This investigation received support from the Filariasis component of the UNDP World Bank WHO Special Programme for Research and Training in Tropical Diseases.