AJTMH HINARI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am. J. Trop. Med. Hyg., 29(3), 1980, pp. 411-415
Copyright © 1980 by The American Society of Tropical Medicine and Hygiene

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hillyer, G. V.
Right arrow Articles by Rossy, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hillyer, G. V.
Right arrow Articles by Rossy, M.

The Enzyme-Linked Immunosorbent Assay (Elisa) for the Detection of Antibodies to DNA

Studies with New Zealand Mice and Murine Schistosomiasis mansoni

George V. Hillyer AND Madeline Rossy
Laboratory of Parasite Immunology, Department of Biology, University of Puerto Rico, Rio Piedras, Puerto Rico 00931

An enzyme-linked immunosorbent assay done with microtiter plates and capable of testing hundreds of sera per day has been developed and evaluated for the detection of antibodies to DNA. An increase of antibody levels with age in New Zealand White mice was demonstrated. We demonstrated for the first time that New Zealand White mice infected with Schistosoma mansoni for 10 weeks have higher anti-DNA antibody levels than controls. We demonstrated that NIH outbred albino mice infected with S. mansoni for 18 weeks developed anti-DNA antibodies by 6 weeks of infection, and found a suggestion that immune complexes are present in the circulation by 9 and 11 weeks of infection. A reasonable correlation between visual end-point titration readings and spectrophotometric readings was found. However, spectrophotometric measurements revealed more subtle changes in antibody concentrations that did visual readings. These results demonstrate the usefulness of the micro-ELISA technique for the detection of anti-DNA antibodies in experimental animals, and warrant further evaluation of this test with the serum of humans with autoimmune diseases such as systemic lupus erythematosus.

Accepted for publication August 11, 1979.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1980 by the American Society of Tropical Medicine and Hygiene.