HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Orjih, A. U. Articles by Nussenzweig, R. S. Search for Related Content PubMed PubMed Citation Articles by Orjih, A. U. Articles by Nussenzweig, R. S.

Immunization against Rodent Malaria with Cryopreserved Irradiated Sporozoites of Plasmodium berghei

The preparation and storage of Plasmodium berghei sporozoites for immunization purposes is described. The sporozoites were harvested from the salivary glands of infected mosquitoes, and maintained in cold tissue culture medium M199 with or without mouse serum. They were irradiated and frozen either at -75°C or in liquid nitrogen. After various periods sporozoites were thawed and injected into A/J mice. At the end of the immunization period the animals were challenged with infective sporozoites of P. berghei and monitored for parasitemia. It was found that the storage did not appreciably alter the ability of the irradiated sporozoites to induce protective immunity in the recipient animals. The highest protection (80–100%) was induced with sporozoites maintained in 10% serum and stored at -75°C.

Accepted for publication December 1, 1979.

This article has been cited by other articles:

				C. Ocana-Morgner, M. M. Mota, and A. Rodriguez Malaria Blood Stage Suppression of Liver Stage Immunity by Dendritic Cells J. Exp. Med., January 20, 2003; 197(2): 143 - 151. [Abstract] [Full Text] [PDF]