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Purification of Giardia Muris Trophozoites by Using Nylon Fiber Columns^*

A method is described for purification of large quantities of Giardia muris trophozoites from the small intestine of the mouse by using density layer centrifugation and nylon fiber columns. Centrifugation of trophozoites obtained from the small intestine on Metrizamide®, specific gravity 1.10, followed by incubation on a nylon fiber column at 37°C for 120 min, yielded up to 15 x 10⁶ viable purified trophozoites per infected mouse.

Accepted for publication June 9, 1979.

^* Supported by U.S. Public Health Service Grant nos. AI-07024 and AI-15351.

Address reprint requests to: Dr. David P. Stevens, Division of Geographic Medicine, University Hospitals, Cleveland, Ohio 44106.