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Am. J. Trop. Med. Hyg., 28(5), 1979, pp. 918-920
Copyright © 1979 by The American Society of Tropical Medicine and Hygiene

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Cultivation of Dengue Virus Type 2 in Baby Hamster Kidney Cells in Serum-Free Medium*

Barbara Malewicz{dagger} AND Howard M. Jenkin
The Hormel Institute, University of Minnesota, Austin, Minnesota 55912

Propagation of dengue virus type 2 (New Guinea C strain) was performed using a shaker culture of baby hamster kidney cells (BHK-21) cultivated in serum-free modified Waymouth medium. Maximum virus titer varied from 108·3 to 108·8 plaque forming units/ml after incubation of BHK-21 cells in suspension culture at 37°C for 40–48 hours post-infection.

Accepted for publication May 5, 1979.


* This investigation was supported in part by U.S. Public Health Service Research Grant HL 08214 from the Program Project Branch, Extramural Programs, National Heart, Lung and Blood Institute; and by The Hormel Foundation.


{dagger} Permanent address: Polish Academy of Sciences, Institute of Oceanology, Sopot, Poland.






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Copyright © 1979 by the American Society of Tropical Medicine and Hygiene.