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Am. J. Trop. Med. Hyg., 28(5), 1979, pp. 845-853
Copyright © 1979 by The American Society of Tropical Medicine and Hygiene

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Antigenic Analyses of Two Axenized Strains of Entamoeba Histolytica by Two-Dimensional Immunoelectrophoresis*

Shu Mei Chang, Chun Mei Lin, Donald G. Dusanic AND John H. Cross
U.S. Naval Medical Research Unit No. 2, Taipei, Taiwan, Republic of China, and Department of Life Sciences, Indiana State University, Terre Haute, Indiana 47809

Antigens in extracts of two strains of Entamoeba histolytica (HT-31 and HK-9) were studied by two-dimensional immunoelectrophoresis (2D-IEP) and 32 and 20 different antigenic components were detected, respectively. Cross-reactions between heterologous systems showed a large number of shared antigens between the two strains. Extracts were fractionated by Sephadex G-200 chromatography. Of the fractions obtained, fraction II of both strains was found to have the most reactive antigenic components. Precipitin patterns of fraction II from HT-31 and HK-9 with sera from patients showed similar 2D-IEP profiles and suggested that in this fraction antigens common to both strains exist which elicit antibody in individuals with E. histolytica infections.

Accepted for publication March 17, 1979.


* This study was supported through funds provided by the Naval Medical Research and Development Command, Navy Department, for Work Unit MR041.09.01-0146.

The research described in this report involved animals maintained in animal care facilities fully accredited by the American Association for Accreditation of Laboratory Animals Care.

The opinions and assertions contained herein are those of the authors and are not to be construed as official or as reflecting the views of the Navy Department or the Naval Service at large.

Address reprint requests to NAMRU-2, Box 14, APO San Francisco, California 96528.






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Copyright © 1979 by the American Society of Tropical Medicine and Hygiene.