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Am. J. Trop. Med. Hyg., 28(3), 1979, pp. 539-547
Copyright © 1979 by The American Society of Tropical Medicine and Hygiene

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Structure of the Schistosome Eggshell: Amino Acid Analysis and Incorporation of Labelled Amino Acids*

J. E. Byram AND A. W. Senft
Departments of Pathology, Harvard Medical School and Peter Bent Brigham Hospital, Boston, Massachusetts 02115, and Division of Biology and Medicine, Brown University, Providence, Rhode Island 02912

Preparations of eggshells of Schistosoma mansoni and S. japonicum were hydrolyzed and analyzed for amino acid composition. Both species showed great similarities in the proportions of each residue found. The predominant amino acid in shell hydrolysates was found to be glycine, which accounted for 37% of S. mansoni and 45% of S. japonicum amino acids. Four components (glycine, aspartic acid, lysine, and serine) totalled 68–75% of amino acids in the eggshells. Other individual amino acids were present in relatively small proportions ranging from 5.2–0.01%. Less than 1% of the amino acid residues were identified as tyrosine, and bityrosine was detected at a level not exceeding 1 in 1,600 residues. Carbohydrates were estimated to comprise 7.5–10% of the eggshell weight, based on hexose assay, and glucosamine was identified as the principal amino sugar in shell hydrolysates. In vivo labelling of the S. mansoni eggshell was demonstrated following injection of C14-glycine and C14-tyrosine into infected mice and subsequent purification of the shells of eggs recovered from their liver.

Accepted for publication September 23, 1978.


* Supported by grants from the U.S.-Japan Cooperative Medical Science Program, NIAID, NIH, the Rockefeller Foundation, and the Edna McConnell Clark Foundation.

Address reprint requests to: Dr. J. E. Byram, Department of Pathology, Peter Bent Brigham Hospital, Boston, Massachusetts 02115.






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Copyright © 1979 by the American Society of Tropical Medicine and Hygiene.