AJTMH Transactions of the Royal Society of Tropical Medicine and Hygiene
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Am. J. Trop. Med. Hyg., 28(2), 1979, pp. 194-197
Copyright © 1979 by The American Society of Tropical Medicine and Hygiene

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Riboflavin Requirement for the Cultivation of Axenic Entamoeba Histolytica*

Hsin-Sheng Lo{dagger} AND Richard E. Reeves{ddagger}
Department of Biochemistry, Louisiana State University Medical Center, 1542 Tulane Avenue, New Orleans, Louisiana 70112

Riboflavin was found to be essential for the cultivation of axenic Entamoeba histolytica. This is the first demonstration of a flavin requirement by the organism. Panmede, the principal source of flavins in the axenic medium, was treated with activated carbon to remove flavins. Medium made with this flavin-deficient Panmede, and supplemented with ribonucleic acid failed to support the multiplication of amebae in serial subculture, but did so when riboflavin was added. The concentration of riboflavin required to achieve maximal growth was about 1.3 µg per ml medium. Studies on riboflavin uptake revealed that amebae lack a high-affinity transport system for this vitamin. The rate of riboflavin uptake was equivalent to the rate of pinocytotic uptake of fluid as previously determined.

Accepted for publication August 26, 1978.


* A preliminary report of this work was presented before the 69th Annual Meeting of the American Society of Biological Chemists in Atlanta, 5–8 June 1978 (Abstract no. 418). Some of the data are from a dissertation by H.-S. Lo submitted to the graduate faculty of the Louisiana State University Medical Center in partial fulfillment of the requirements of the Doctor of Philosophy degree, 1978. Supported, in part, by Grant AI-02951 from the National Institutes of Health.


{dagger} Holder of a fellowship from the National Science Council, Republic of China. Present address: Department of Parasitology, National Defense Medical Center, P.O. Box 8244, Taipei, Taiwan, Republic of China.


{ddagger} To whom reprint requests should be addressed.






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