HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Takahashi, M. Articles by Suzuki, K. Search for Related Content PubMed PubMed Citation Articles by Takahashi, M. Articles by Suzuki, K.

Japanese Encephalitis Virus in Mosquito Salivary Glands

Culex tritaeniorhynchus and C. pipiens mosquitoes were infected with Japanese encephalitis virus either by intrathoracic injection or by membrane feeding. The virus maturation sites and the process of virus particle concentration in salivary gland cells were studied by electron microscopy. Occurrence of mature virions was primarily associated with intracytoplasmic viral matrices which were extraordinarily large and had a perinuclear location in c. pipiens mosquitoes. The other sign of virus replication was the proliferation of small spherical vesicles throughout the cytoplasm. It appeared that mature virions were entrapped in intracellular vacuoles and later released into the apical cavity of salivary gland cells through the fusion of these vacuoles with the apical plasma membrane. This process seemed to be associated with primary resynthesis of saliva in mosquitoes following blood feeding activity. Another type of shedding involved virus particles either singly or in mass being released directly through the apical plasma membrane. All of these events occurred only in cells of the lateral lobes of the salivary glands, which fact was confirmed by immunofluorescent staining of infected glands. The median lobe of mosquito salivary glands may have a minor or no role in the transmission of Japanese encephalitis virus.

Accepted for publication May 27, 1978.

This article has been cited by other articles:

				Y. A. GIRARD, B. S. SCHNEIDER, C. E. MCGEE, J. WEN, V. C. HAN, V. POPOV, P. W. MASON, and S. HIGGS SALIVARY GLAND MORPHOLOGY AND VIRUS TRANSMISSION DURING LONG-TERM CYTOPATHOLOGIC WEST NILE VIRUS INFECTION IN CULEX MOSQUITOES Am J Trop Med Hyg, January 1, 2007; 76(1): 118 - 128. [Abstract] [Full Text] [PDF]