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Release of Romanomermis Culicivorax for the Control of Anopheles Albimanus in El Salvador

I. Mass Production of the Nematode

The mosquito parasite, Romanomermis culicivorax, was mass produced for the treatment of 144,000 m² of Anopheles albimanus breeding area in El Salvador. The production of the necessary inoculum required the exposure of 1.6 x 10⁶ first-instar Culex p. quinquefasciatus larvae to 137 x 10⁶ preparasites (1:14 ratio) each week for 6 weeks. The method employed produced an average 13.7 g (ca. 2,200/g) of postparasitic nematodes per rearing tray (20,000 mosquitoes), a total of 6,392 g (14.1 lb) for the 6-week period, and 425 cultures.

Accepted for publication April 8, 1978.