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Identification and Antigenic Typing of Rickettsia Tsutsugamushi in Naturally Infected Chiggers (Acarina: Trombiculidae) by Direct Immunofluorescence^*

A. L. Dohany, A. Shirai, D. M. Robinson, S. Ram AND D. L. Huxsoll

Rickettsia tsutsugamushi organisms were detected and typed antigenically by direct immunofluorescence in mites from laboratory-maintained infected colonies of Leptotrombidium (Leptotrombidium) fletcheri and L. (L.) arenicola. Rickettsiae were identified most readily in unengorged larvae, but were also discernable in engorged larvae and all post-larval stages of the vectors.

Accepted for publication April 8, 1978.

^* This work was supported by Research Grant No. DAMD17-77-G-9426 from the U.S. Army Medical Research and Development Command, Washington, D.C. 20314.

Present address: Walter Reed Army Institute of Research, Walter Reed Army Medical Center, Washington, D.C. 20012.