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Growth of Babesia Bovis in Bovine Erythrocyte Cultures^*

Babesia bovis was cultured in a suspension of bovine erythrocytes incubated at 37°C in Medium 199 with 50% bovine serum. The cells in culture were kept in suspension by slow stirring in spinner flasks and the medium was replaced at 24-hour intervals. Persistent multiplication of the parasite in a short series of subcultures suggests the feasibility of this approach for continuous culture.

Accepted for publication June 10, 1978.

^* This report is the result of a joint research and training program in hemotropic diseases between the College of Veterinary Medicine of the University of Illinois and the Instituto Nacional de Investigaciones Pecurias (INIP) supported by funds of the Mexican Department of Agriculture, the University of Illinois, and The Rockefeller Foundation (research grant RF-44-44-30-362), New York, N.Y.

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