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Oubangui (OUB)

Strain: Ar B 3816

Information from: J. P. Digoutte, Institut Pasteur, B.P. 304, Cayenne, French Guiana, 30 May 1975.

Antigenic group: Ungrouped.

Original source: Isolated by J. P. Digoutte and M. Germain at Institut Pasteur, Bangui, from pool of female Culex guiarti mosquitoes collected on 26 April 1972 by use of human bait at Bangui, Central African Republic, Let. 4°22'N, Long. 18°35'E. Habitat: Park of the Institut Pasteur in the town of Bangui. Storage: Stored in electrical deep freezer at -75°C.

Method of isolation in laboratory: Mosquitoes ground in Earle's balanced salt solution containing bovine albumin and antibiotics.

Validity of isolation: Reisolation not tried but first virus of this type in the laboratory.

Properties of virus: Physical: Virus filterable through Millipore 220 nm. Chemical: No data. Resistance to chemicals: Sensitive to chloroform; control titer 10^5.5 after-treatment titer <10^2.0. Antigenic: Hemagglutinin detected in test with infected suckling mouse brains extracted with sucrose-acetone and tested with goose erythrocytes at pH 5.7 through 6.6 and at room temperature. Optimal pH was 6.2.