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Information from: T. Omori, National Institute of Animal Health, Kodaira, Tokyo, Japan, 5 March 1976.
Antigenic group: Ungrouped.
Original source: Isolated by National Institute of Animal Health at Ibaraki Prefecture, Japan from blood of 10-yr-old cow manifesting fever, anorexia, lacrimation, deglutitive difficulty, and ulceration of oral-nasal mucosa. Sample collected on 20 September 1959 at Ibaraki Prefecture, central parts of Japan, Let. 37°N, Long. 140°E. Habitat: Open ricefield. Storage: Not stated.
Methods of isolation in laboratory: Blood defribrinated and 0.1 ml inoculated into primary bovine embryo kidney (BEK) cell cultures on 20 September 1959. Cell cultures maintained with YLH, Hanks' solution,0.5% lactalbumin hydrolysate, 0.1% yeast extract, and 10% calf serum. CPE observed after 1 blind passage.
Validity of isolation: Virus reisolated.
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