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Information from: R. Ackermann and B. Rehse-Küpper, Department of Virology, Neurology Clinic, University of Cologne, Germany, 6 June 1975.
Antigenic group: Colorado Tick Fever.
Original source: Isolated by B. Rehse-Küpper at Department of Virology,Neurology Clinic, University of Cologne, from a pool of 2 adult male and 2 adult female Ixodes ricinus (L.) ticks collected 13 May 1972 by flagging the vegetation at Eyach, B.-W., West Germany, Lat.48°27'N, Long. 8°41'E. Habitat: Mixed woodland area. Storage: Held in glass tube at 4°C until inoculation.
Methods of isolation in laboratory: Ticks ground in 0.03 M tris buffer, pH 7.5 with 10% calf serum. Inoculated 0.02 ml ic + sc in 2-day-old mice on 6 June 1972
Validity of isolation: Virus not reisolated. but first virus of this type in laboratory.
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