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Am. J. Trop. Med. Hyg., 27(2), 1978, pp. 365-368
Copyright © 1978 by The American Society of Tropical Medicine and Hygiene

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NAD Nucleosidase of Agkistrodon Bilineatus Venom

E. M. Brunson, B. D. Johnson AND D. H. Sifford
College of Science, Arkansas State University, State University, Arkansas 72467

NAD nucleosidase (NAD glycohydrolase, EC 3.2.2.5) activity in Agkistrodon bilineatus venom was observed. Using the cyanide assay method at pH 7.9, lyophilized crude venom had an activity of 0.19 units/mg. Chromatography of the crude venom on DEAE Sephadex A-50 with ammonium acetate buffer by two stage elution yielded 13 fractions. Peak NAD nucleosidase activity occurred at Fraction X. Crude venom and Fraction X NAD nucleosidase activities were thermolabile.

Accepted for publication July 23, 1977.






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Copyright © 1978 by the American Society of Tropical Medicine and Hygiene.