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Laboratory Studies of Transovarial Transmission of Trivittatus Virus by Aedes Trivittatus^*

Aedes trivittatus were experimentally exposed through a membrane to a concentration of trivittatus (TVT) virus in dog defibrinated blood of 10^3.4–10^3.5 SMICLD₅₀/0.025 ml. Transovarial and transstadial transmission was demonstrated. Fifty-five percent of the infected mosquitoes tested transovarially transmitted TVT virus. TVT virus was isolated from 16% of the F₁ generation progeny examined, including 19% of the larvae, 23% of adult females, and 10% of adult males.

Accepted for publication July 23, 1977.

^* Journal Paper No. J-8789 of the Iowa Agriculture and Home Economics Experiment Station, Ames, Iowa 50011. Project No. 2053.