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Germfree Biomphalaria glabrata were successfully reared through their life cycle in isolators. The snails were fed a diet sterilized either by heat or by irradiation. Snails fed an irradiated diet had a better growth and higher reproduction rate than the ones fed an autoclaved diet. There was no difference in survival between the two groups.
Accepted for publication December 18, 1976.
Parts of this work were reported at the annual meeting of the Association for Gnotobiotics, Notre Dame, Indiana, July 1976.
* This work was supported by the following grants: GA BMS 7305 from The Rockefeller Foundation; 199 from Banco Nacional de Desenvolvimento Econômico (FUNTEC); and SIP/08-096 from Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq).
Fellow of Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq). Present address: Centre de Pesquisas René Rachou, Avenida Augusto de Lima, 1715, Belo Horizonte, Minas Gerais, Brazil.
Fellow of Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq).
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