HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Fauvel, M. Articles by Spence, L. Search for Related Content PubMed PubMed Citation Articles by Fauvel, M. Articles by Spence, L.

Immune Electron Microscopy of Arboviruses^*

M. Fauvel, H. Artsob AND L. Spence

Representative members of four arbovirus taxons were studied by immune electron microscopy (IEM) in an attempt to determine whether the technique can be applied to the specific identification of arboviruses. Alphavirus, flavivirus, bunyavirus, and rhabdovirus antigens were prepared by propagation in tissue culture. The viruses were used to titrate homologous antibody and test heterologous antibody in the IEM test. Specific clumping together with visualization of antibody attachment was observed for all homologous systems studied. For the alphaviruses, titers of antibody, as determined by IEM, were comparable with the titers obtained by hemagglutination inhibition. Alphavirus cross-reactions were observed at low antibody dilutions by IEM. However, further dilution of antibody resulted in specific clumping only of homologous virus, allowing specific identification of alphavirus unknowns. For all viruses studied, positive IEM reactions were obtained with homologous grouping ascitic fluids but not with heterologous grouping fluids.

Accepted for publication December 31, 1976.

^* Part of this investigation was supported by grants from the Medical Research Council.

Part of this investigation was undertaken at the Department of Microbiology and Immunology, McGill University, in partial fulfillment of the requirements for the degree of Master of Science.

National Arbovirus Reference Service. A laboratory operating in conjunction with the Laboratory Centre for Disease Control, Department of National Health and Welfare, Canada.

This article has been cited by other articles:

				W. W. S. I. Rodrigo, X. Jin, S. D. Blackley, R. C. Rose, and J. J. Schlesinger Differential Enhancement of Dengue Virus Immune Complex Infectivity Mediated by Signaling-Competent and Signaling-Incompetent Human Fc{gamma}RIA (CD64) or Fc{gamma}RIIA (CD32). J. Virol., October 1, 2006; 80(20): 10128 - 10138. [Abstract] [Full Text] [PDF]