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Am. J. Trop. Med. Hyg., 26(3), 1977, pp. 533-537
Copyright © 1977 by The American Society of Tropical Medicine and Hygiene

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A Simple Technique for the Detection of Dengue Antigen in Mosquitoes by Immunofluorescence

Timothy T. Kuberski AND Leon Rosen
Pacific Research Section, Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Honolulu, Hawaii 96806

Dengue antigen could be demonstrated by a direct fluorescent antibody technique in simple head squashes of either male or female Aedes albopictus mosquitoes infected with any of the four dengue serotypes. Dengue viruses can be isolated rapidly and simply by combining this technique with that of parenteral inoculation of mosquitoes with test material.

Accepted for publication October 16, 1976.




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Copyright © 1977 by the American Society of Tropical Medicine and Hygiene.