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Enzyme- and ¹²⁵I-Labeled Anti-Immunoglobulin Assays in the Immunodiagnosis of Schistosomiasis^*

An enzyme-linked immunosorbent assay (ELISA), and radioimmunoassays using antigen bound to plastic tubes or microcrystalline particles, were adapted for the indirect serological measurement of anti-schistosome antibodies. These alkaline phosphatase and ¹²⁵I-labeled anti-immunoglobulin techniques were found to be highly quantitative and adaptable. Comparison showed them to be considerably more sensitive than the indirect hemagglutination and indirect fluorescent antibody techniques. Using adult S. mansoni freeze-thaw antigen, anti-schistosome antibodies were detected in sera from patients infected with Schistosoma mansoni and S. haematobium. However, results in patients with schistosomiasis haematobium were generally intermediate between those of patients with schistosomiasis mansoni and normal, uninfected controls.

Accepted for publication March 27, 1976.

^* Supported by the Naval Medical Research and Development Command, Work Unit No. MF51.524.009.0006, and ONR Contract No. N0014-70-C-0331 to the American Foundation for Biological Research, Rockville, Maryland. The opinions or assertions contained herein are the private ones of the authors and are not to be construed as official or reflecting the views of the Navy Department or the naval service at large. The animals used in this study were handled in accordance with the provisions of Public Law 89-54 as amended by Public Law 91-579, the "Animal Welfare Act of 1970," and the principles outlined in the "Guide for the Care and Use of Laboratory Animals," U. S. Department of Health, Education, and Welfare publication no. (NIH) 73-23.