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Study of the Circumoval Precipitate in Schistosomiasis Japonica by Immunofluorescent Technic^*

The 48-hour circumoval precipitates formed by infected human and rabbit sera on lyophilized Schistosoma japonicum eggs were stained with the corresponding fluorescein-labelled monospecific goat antisera for study under a fluorescent microscope. The three major Ig classes were found to participate in the formation of the precipitate, with major components being IgG and IgM; IgA participated least. IgE and C3 also contributed to the precipitate. The circumoval precipitate on S. japonicum eggs is therefore a complex of different immunoglobulins reactive with antigen(s) from the egg.

Accepted for publication October 25, 1975.

^* This study was supported by the U.S.-Japan Cooperative Medical Science Program administered by the National Institute of Allergy and Infectious Diseases of the USPHS under Grant No. AI 07724.