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Evaluation of Whole and Purified Hydatid Fluid Antigens in the Diagnosis of Human Hydatidosis by the Immunoelectrophoresis Test^*

V. M. Varela-Díaz, J. A. Guisantes, M. I. Ricardes, L. A. Yarzábal AND E. A. Coltorti

Whole sheep hydatid cyst fluid (WHF) and purified hydatid fluid antigens (PHF) were evaluated in the immunoelectrophoresis (IEP) test for the diagnosis of human hydatid disease. A higher sensitivity was obtained using the PHF than with the WHF antigen but false positive results were observed with the former in 22.4% of the 102 non-hydatid sera examined. Using the presence of arc 5 as the criterion of positivity however, no false positives were observed in non-hydatid sera with the WHF antigen. It is postulated that the number of bands other than arc 5 may be of value in the diagnosis of hydatid disease by the IEP test, in cases where this diagnostic arc is absent.

Accepted for publication July 13, 1974.

^* Address reprint requests to: Pan American Zoonoses Center, Casilla 23, Ramos Mejía, Argentina.

Fellow, Pan American Health Organization.

Short-term consultant, Pan American Health Organization.