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Am. J. Trop. Med. Hyg., 23(6), 1974, pp. 1197-1202
Copyright © 1974 by The American Society of Tropical Medicine and Hygiene

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The Methylene Blue Reduction Test: Evaluation of a Screening Method for Glucose-6-Phosphate Dehydrogenase Deficiency

W. N. Gibbs
Department of Pathology, University of the West Indies, Kingston 7, Jamaica

Male and female neonates, children and adults, and patients with hemolytic anemia, in whom subnormal erythrocyte glucose-6-phosphate dehydrogenase deficiency was demonstrated on spectrophotometric assay, were all detected using the methylene blue reduction screening test. This finding and the results of mixing experiments suggested that it is as sensitive as the assay. Offering advantages over other screening tests, it is useful for surveys and for routine laboratory work.

Accepted for publication April 6, 1974.






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Copyright © 1974 by the American Society of Tropical Medicine and Hygiene.