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Infection and Transmission of Eastern Equine Encephalomyelitis Virus with Colonized Culiseta Melanura (Coquillett)^*

John J. Howard AND Robert C. Wallis

Colonized Culiseta melanura were infected with eastern equine encephalitis (EEE) virus by feeding on viremic 1-day-old chicks. The approximate minimal mosquito infective dose was 10⁴ baby mouse LD₅₀/0.02 ml of blood, the approximate 50% dose was 10⁵, and the approximate 90% dose was 10⁶ LD₅₀/0.02 ml. Over 85% of infected mosquitoes transmitted EEE virus to baby chicks after a 2-week extrinsic incubation.

Accepted for publication November 2, 1973.

^* This study was conducted as partial fulfillment of the requirements for the degree of Doctor of Public Health from Yale University. It was supported in part by USPHS Training Grant No. 2T01-GM 00005-16EBA, by USPHS Grant No. AI-10984, and in part by Yale University.

Present address: New York State Department of Health, 1475 Winton Road North, Rochester, New York 14609.

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