AJTMH ASTMH Job Mart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am. J. Trop. Med. Hyg., 23(3), 1974, pp. 420-428
Copyright © 1974 by The American Society of Tropical Medicine and Hygiene

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Dean, D. A.
Right arrow Articles by Murrell, K. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dean, D. A.
Right arrow Articles by Murrell, K. D.
Right arrowPubmed/NCBI databases
*Substance via MeSH

Combined In Vitro Effects of Rat Antibody and Neutrophilic Leukocytes on Schistosomula of Schistosoma Mansoni*

David A. Dean, Richard Wistar AND K. Darwin Murrell
Experimental Parasitology Division and Experimental Immunology Division, Naval Medical Research Institute, National Naval Medical Center, Bethesda, Maryland 20014

Schistosomula of Schistosoma mansoni were shown to be rapidly killed in vitro by the combined effects of rat neutrophilic leukocytes, IgG antibodies from schistosome-infected rats, and heat-labile factors found in normal serum. A microassay system was developed for characterizing and quantitating this opsonization reaction. Following a single infection, serum opsonin levels reached a peak at approximately 6 weeks and then decreased only slightly until the 18th week post-infection. In the presence of antiserum of high titer, 100% of schistosomula were heavily ensheathed and apparently killed by neutrophils within 20 hours. Ensheathed schistosomula stained heavily with nitroblue tetrazolium dye, indicating the release of lytic enzymes by the neutrophils. Both ensheathment and killing were prevented if normal serum was used in place of antiserum, or if neutrophils were removed from the leukocyte suspension.

Accepted for publication October 27, 1973.


* Supported by the Bureau of Medicine and Surgery Work Unit No. MR041.05.01.0023A6GI.

The opinions or assertions contained herein are the private ones of the author and are not to be construed as official or reflecting the views of the Navy Department or the naval service at large.

The experiments reported herein were conducted according to the principles outlined in the Animal Welfare Act (PL 89–544 as amended) and followed the guidelines prescribed in DHEW Publication No. (NIH) 72–23, formerly PHS Publication No. 1024, "Guide for Laboratory Animal Facilities and Care."






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1974 by the American Society of Tropical Medicine and Hygiene.