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Am. J. Trop. Med. Hyg., 23(3), 1974, pp. 350-354
Copyright © 1974 by The American Society of Tropical Medicine and Hygiene

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Leishmania in the Chick Embryo

III. Multiplication of L. mexicana and L. braziliensis in the Liver*

Norman R. Dollahon{dagger} AND Thomas W. Holbrook
Department of Zoology and Bureau of Biological Research, Rutgers University, New Brunswick, New Jersey 08903

Promastigotes of Panamanian strains of Leishmania mexicana and L. braziliensis were inoculated intravenously into 14-day chick embryos. The course of infection was followed by examination of liver impression smears prepared from embryos incubated at 28, 33, and 35°C for 1 hour and 1, 2, and 4 or 6 days post-infection. Parasites of each species declined in numbers rapidly at 35°C. Parasites multiplied more rapidly at 28 than at 33°C, and greater numbers of parasites were observed in the liver after 4 days of incubation at 28°C than after 6 days at 33°C. Multiplication of the two Leishmania species was similar at 28 and 33°C, respectively. Morphologically, L. braziliensis in the liver of chick embryos was more compact and stained more intensely than L. mexicana. Although L. mexicana was generally pale-staining and swollen in appearance, the parasites were viable as demonstrated in cultures initiated with infected liver. Promastigote and amastigote forms were observed in embryos incubated at 28°C, while no flagellum was seen on forms at 33°C.

Accepted for publication October 27, 1973.


* Supported by Training Grant AI-00187, Research Grant AI-00092, and in part by Research Grant AI-08988, National Institute of Allergy and Infectious Diseases, U. S. Public Health Service, and by the Charles and Johanna Busch Memorial Fund Award to the Bureau of Biological Research, Rutgers University.


{dagger} Presently at the Department of Biology, Villanova University, Villanova, Pennsylvania 19085.






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Copyright © 1974 by the American Society of Tropical Medicine and Hygiene.