HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Dunsford, H. A. Articles by Von Lichtenberg, F. Search for Related Content PubMed PubMed Citation Articles by Dunsford, H. A. Articles by Von Lichtenberg, F.

Artificial Granulomas from Bentonite and Latex Carrier Particles^*

The experimental granuloma system has been further studied by examination of soluble schistosome antigen (SEA) coated bentonite granulomas at 8 and 16 days, at which time epitheloid cells and giant cells were observed confirming the ability of SEA to elicit a true granuloma. The elicitation of delayed hypersensitivity by SEA has been studied in both the mouse foot pad and coated on bentonite particles and latex beads in the mouse lung. In the foot pad SEA elicited an early acute inflammatory response followed by delayed hypersensitivity type dermal response in the sensitized mouse lasting 2 to 4 days. SEA adsorbed to bentonite particles or latex beads elicited a histologically typical granulomatous response in the sensitized mouse lung which peaked at 2 to 4 days and lasted 16 or 8 days respectively. Thus cell response to the same antigen in the same animal differed according to whether it was immediately diffused or was gradually released from a carrier particle, and the duration of the granuloma was proportional to the antigen uptake by the particle. Although SEA release from particles was virtually complete within 60 minutes in vitro, antigen persisted in lung granulomas for at least 24 hours as shown by immunofluorescence. The elicitation of delayed hypersensitivity by SEA was also demonstrated by macrophage inhibition in a micro method using homologous mouse peritoneal macrophages. These findings agree with the hypotheses that delayed hypersensitivity and antigen sequestration are involved in schistosome granuloma formation.

Accepted for publication August 25, 1973.

^* Supported by Grant 5 R 22 AI 02631 of the National Institutes of Health, and the U.S.-Japan Cooperative Medical Science Program, and by Contract DADA 17-72-C-2056 of the Office of The Surgeon General, Armed Forces Epidemiological Board.

Address reprint requests to: Dr. Franz v. Lichtenberg, Department of Pathology, Harvard Medical School, 25 Shattuck Street, Boston, Massachusetts 02115.