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Use of Stable Sensitized Cells in Indirect Microhemagglutination Test for Malaria^*

Human "O" erythrocytes were stabilized with pyruvic aldehyde, tanned, and fixed with glutaraldehyde. These cells were sensitized with antigenic preparations of Plasmodium knowlesi and were used in the indirect microhemagglutination test with serum samples from human malaria cases and negative controls. The cells did not lose sensitivity after 3 months of storage. The practical application of these cells in seroepidemiological studies of malaria is pointed out.

Accepted for publication June 3, 1972.

^* This study was supported in part by the U. S. State Department, Agency for International Development, Participating Agency Service Agreement (PASA) No. RA (HA) 5–68.