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Passive Hemagglutination Inhibition Technique for the Identification of Arthropod Blood Meals^*

A passive hemagglutination inhibition (PHI) technique to identify arthropod blood meals is described. The antisera for this test were prepared in chickens using chemically purified antigens. The antibody obtained was purified by immunoadsorption. The blood meals of several laboratory-fed, as well as several hundred field-collected mosquitoes were identified by this test. These field-collected mosquitoes were previously identified by the precipitin test as feeding on Leporids, Ruminants or Passeriformes. More definitive host identifications were obtained using the PHI test.

Accepted for publication October 16, 1971.

^* Research supported in part by the U. S. Army Medical Research and Development Command under Contract No. DADA17-70-6-0166; and General Research Support Grant I-S01-FR05441 from the National Institutes of Health, U. S. Department of Health, Education, and Welfare.