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Biochemical Characterization and Skin Test Sensitivity of Mycelial Growth Filtrate of Histoplasma Duboisii^*

Sunday O. Fadulu AND Howard W. Larsh

A controlled study of the biochemical characteristics and potency of skin-test antigens was conducted on crude mycelial filtrates (1–24 weeks old) of two isolates of Histoplasma duboisii. Results of the study indicate that mycelial filtrates of H. duboisii may be specific for the detection of allergies created by this organism. A mycelial filtrate of Histoplasma capsulatum, H-42, gave a complement-fixation reaction with serum of guinea pigs infected with H. duboisii. Also, immunoelectrophoretic studies indicate that H. duboisii and H. capsulatum have at least one antigen in common, but that the "M" antigen of H. capsulatum differs from that of H. duboisii. The crude mycelial filtrate may be fractionated by ion-exchange chromatography, producing materials which are useful in skin tests. The characteristic development of severe orchitis in male guinea pigs was also observed following intraperitoneal injections of large doses of mycelial fragments and yeast cells of H. duboisii.

Accepted for publication September 9, 1971.

^* The data in this paper were taken from the dissertation submitted by the senior author in partial fulfillment of the requirements for the Ph.D. degree.

Present address: University Ife, Department of Biological Sciences, Ile-Ife, Nigeria.