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Morphology and Infectivity of Leishmania Donovani Cultivated in Nonliving Media at Elevated Temperatures^*

Teferra Wonde AND B. M. Honigberg

Leishmania donovani strain 3S (Malakal area, Sudan) was adapted gradually to growing at temperatures that ranged from 30° to 37°C. Rich cultures of the parasites could be maintained by serial transfers at the various temperatures in modified Tobie's medium. Growth of L. donovani at temperatures exceeding 32°C occurred only in media supplemented with chick-embryo extract. At all temperatures, cultures of fully-adapted, vigorous organisms consisted primarily of typical promastigote stages. Flagellates grown at the several temperatures retained infectivity for hamsters as well as for cultures of L, human heart, human liver, and HeLa cell lines.

Accepted for publication March 12, 1971.

^* This investigation was supported by Research Grants AI00742-15, and AI00742-16 from the National Institute of Allergy and Infectious Diseases.

Please address requests for reprints to Dr. B. M. Honigberg, Department of Zoology, Morrill Science Center, University of Massachusetts, Amherst, Massachusetts 01002.

Dr. Wonde was a Research Associate in the Department of Zoology, University of Massachusetts at Amherst, from August 1969 to September 1970. Present address: Imperial Central Laboratory and Research Institute, Addis Ababa, Ethiopia.