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Susceptibility of Tissue Cultures of Minced Aedes Aegypti Pupae and Adults to Infection with Venezuelan Equine Encephalomyelitis Virus^*

The Trinidad strain of Venezuelan equine encephalomyelitis virus was propagated successfully in tissue cultures of germ-free, minced Aedes aegypti pupae. Peak virus titers of 5.0 to 6.0 log₁₀ plaque-forming units per ml were reached within 3 to 4 days after cultures were infected. Five serial passages caused no detectable changes in either mouse virulence or plaque-size characteristics of the virus. An attenuated virus strain derived from the Trinidad strain did not multiply in these cultures, and neither virus strain grew in tissue cultures of germ-free minced adult mosquitoes.

Accepted for publication March 12, 1971.

^* Conducting the research reported herein, the investigators adhered to Guide for Laboratory Animal Facilities and Care established by the Committee on the Guide for Laboratory Animal Facilities and Care of the Institute of Laboratory Animal Resources, NAS-NRC.