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Demonstration of Venezuelan Equine Encephalomyelitis Virus in Tissues of Aedes Aegypti^*

Aedes aegypti were fed on hanging drops containing Venezuelan equine encephalomyelitis (VEE) virus and their tissues were studied for the presence of virus by electron-microscopy. Virus penetrated the digestive tract and reached the brain within 48 hours after the infective feeding. It was not found in the eyes. It was found in the ovaries, but not within follicle cells or eggs, in the tissues of the Malpighian tubules, but not in their lumina. Although VEE virus appears to be pantrophic in tissues of A. aegypti, it showed a preference for the salivary glands, where it was markedly concentrated by the 8th day after infection.

Accepted for publication March 25, 1971.

^* This work was supported by contract AMC 197A with the Department of the Army, Fort Detrick, Frederick, Maryland 21701.

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