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No. 226, Dugbe (DUG)

Strain: AR 1792

Information from: O. R. Causey, University of Ibadan, Ibadan, Nigeria, 31 August 1968.

Antigenic group: Ganjam.

Original source: Isolated by Virus Research Laboratory, University of Ibadan, Ibadan, Nigeria, from adult male Amblyomma variegatum picked by hand from cattle on 14 and 15 October 1964 at Ibadan, Nigeria, Lat. 7° 20' N, Long. 3° 50' E. Habitat: White Fulani cattle at cattle market. Between Guinea woodland and rain forest vegetation zones. Storage: Ticks stored in -70°C Revco until inoculated into mice.

Method of isolation in laboratory: Ground suspension in bovine albumin in phosphate-buffered saline solution. Inoculated in 4-day-old mice 0.02 ml ic on 16 October 1964.

Validity of isolation: Numerous isolations from tick pools and cattle blood, and specific antibody in associated animals.

Properties of virus: Physical: Filterable through Seitz EK filter pad. Chemical: No data. Resistance to chemicals: Sensitive to 1:1,000 sodium deoxycholate; control titer 10^7.4, aftertreatment titer 10^3.5. Sensitive to 1:100 chloroform; control titer 10^5.9, after-treatment titer 10^1.5.