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No. 206, Manawa (MWA)

Strain: Argas T-461

Information from: H. C. Barnett, Pakistan Medical Research Center, 6, Birdwood Road, Lahore, West Pakistan,^* 31 May 1967.

Antigenic group: Uukuniemi.

Original source: Isolated by J. R. Gorham at Lahore, West Pakistan, from pool of adult male and female Argas abdussalami caught by hand on 7 August 1964 at Manawa, 13 miles east of Lahore, Lat. 31° 32' N, Long. 74° 30' E. Habitat: Dalbergia trees where Gyps bengalensis roost, alluvial plain of Indus River, elevation 800 ft, climate hot and dry. Storage: Ticks held alive until inoculated into mice.

Method of isolation in laboratory: Ground suspension diluted in bovine albumin in phosphate-buffered saline solution, pH 7.2. Inoculated 0.01 ml ic in 1-day-old mice on 21 September 1964.

Validity of isolation: Reisolation not tried.

Properties of virus: Physical: No data. Chemical: No data. Resistance to chemicals: Sensitive to 1:1,000 sodium deoxycholate (after-treatment titer 10^2.9 LD₅₀) and chloroform (after-treatment titer 10^2.9 LD₅₀); control titer >10^7.0 LD₅₀.

^* Present address: H. C. Barnett, University of Maryland School of Medicine, Division of Medical Entomology and Ecology, 29 So. Greene Street, Baltimore, Maryland 21201.