HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Senna, I. A. Articles by Vieira, E. C. Search for Related Content PubMed PubMed Citation Articles by Senna, I. A. Articles by Vieira, E. C.

Culture of the Snail Biomphalaria Glabrata on a Simplified Medium^*

Ioni A. Senna AND Enio Cardillo Vieira

To promote growth and reproduction of axenic Biomphalaria glabrata, we have devised a simplified and purified nutrient medium. This diet differed from a previously described casein-lettuce medium in that powdered lettuce was omitted and cellulose, pectin, starch, glucose, ascorbic acid, and cysteine were added. The liquid portion was the same as in the casein-lettuce medium. Thirty-eight axenic snails were fed on each medium; after 5 months there were 14 snails on the casein-lettuce medium, 18 on the simplified medium. The snails did not differ in size on either medium, but snails eating the simplified medium laid fewer eggs than those eating the casein-lettuce medium. Age of snails at onset of oviposition was the same for each medium.

Accepted for publication September 19, 1969.

^* This work was supported by United States Public Health Service through grant AI-08245 and by Conselho Nacional de Pesquisas through grant TC-7853. Part of the permanent equipment was a gift of The Rockefeller Foundation.

Pesquisador chefe from Conselho Nacional de Pesquisas.