AJTMH Transactions of the Royal Society of Tropical Medicine and Hygiene
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Am. J. Trop. Med. Hyg., 19(1), 1970, pp. 49-56
Copyright © 1970 by The American Society of Tropical Medicine and Hygiene

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Blast Transformation and Migration-Inhibition in Toxoplasmosis and Leishmaniasis*,{dagger},

Lawrence Tremonti{ddagger} AND Bryce C. Walton§
Department of Medicine, University of Illinois College of Medicine, Box 6998, Chicago, Illinois 60680 and U.S. Army Medical Research Unit Component, Middle America Research Unit, Box 2011, Balboa Heights, Canal Zone

The technique of blast transformation in naturally occurring disease in man, and that of macrophage migration-inhibition in experimentally induced infections in animals, were applied to the study of cellular hypersensitivity in Toxoplasma gondii and Leishmania braziliensis infections. Blast transformation of peripheral lymphocytes of patients with American cutaneous leishmaniasis occurred in the presence of leishmanin at a rate of 2% or greater, but there was no significant transformation in noninfected controls. However, in both Toxoplasma-infected patients and presumably negative controls, Toxoplasma antigens stimulated blast transformation. With macrophage inhibition, the converse relation was seen, with the more striking results obtained in the experimental Toxoplasma infections. Inhibition of macrophage migration in the presence of antigen was pronounced in five of seven guinea pigs, but only one of seven Leishmania-inoculated animals showed evidence of inhibition by leishmanin. This was correlated with development of strong skin-test reactions in the Toxoplasma animals, and only slight reactions to the Leishmania. Utilization of these in vitro immunologic techniques should be of value for further study of host response to parasitic infections.


* This investigation was supported in part by Public Health Service training grant TOI A1 0020B from the Institute of Allergy and Infectious Diseases, National Institutes of Health.


{dagger} The "Guide for Laboratory Animal Facilities and Care," published by the Committee for the Guide for Laboratory Animal Resources, National Academy of Sciences, National Research Council, provided the principles for the conduct of the animal experiments reported herein.


{ddagger} Former Trainee in Infectious Diseases, University of Illinois College of Medicine, Visiting Scientist, MARU.


§ Lt. Col. MSC, USA. Chief, Parasitic Diseases Section, MARU.






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