AJTMH HINARI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am. J. Trop. Med. Hyg., 19(1), 1970, pp. 119-122
Copyright © 1970 by The American Society of Tropical Medicine and Hygiene

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Maire, L. F.
Right arrow Articles by Cole, F. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Maire, L. F., III
Right arrow Articles by Cole, F. E., Jr.

An Inactivated Eastern Equine Encephalomyelitis Vaccine Propagated in Chick-Embryo Cell Culture

I. Production and Testing*,{dagger},

Louis F. Maire, III, Robert W. McKinney AND Francis E. Cole, Jr.
U. S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, Maryland 21701

A formalin-inactivated Eastern equine encephalomyelitis vaccine was prepared from the PE-6 strain virus, propagated in primary chick-embryo cell cultures maintained with Medium 199 containing 0.25% human serum albumin. To effect an increase in antigenic mass per unit volume of fluid harvest, we used the pooled fluids from the first tissue-culture passage as maintenance medium for two additional passages. A formalin concentration of 1:2,000 (0.05%) at 37°C was used for inactivation of the virus suspension. Tests on the "in-process" and final vaccine documented the safety and sterility. Tests of potency on the final lyophilized product in hamsters and guinea pigs yielded ED50 values of 0.009 ml and 0.012 ml, respectively. Recent assays have revealed no change in potency after storage of the final product for 17 months at -20°C.


* These studies were conducted in conjunction with a program for development and testing of vaccines for, and the therapy of, acute infection. The investigations were supervised by the Commission on Epidemiological Survey of the Armed Forces Epidemiological Board.


{dagger} Requests for reprints should be addressed to Dr. Cole.




This article has been cited by other articles:


Home page
 Am J Trop Med HygHome page
N. C. Arrigo, D. M. Watts, I. Frolov, and S. C. Weaver
Experimental Infection of Aedes sollicitans and Aedes taeniorhynchus with Two Chimeric Sindbis/Eastern Equine Encephalitis Virus Vaccine Candidates
Am J Trop Med Hyg, January 1, 2008; 78(1): 93 - 97.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1970 by the American Society of Tropical Medicine and Hygiene.