AJTMH Transactions of the Royal Society of Tropical Medicine and Hygiene
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am. J. Trop. Med. Hyg., 18(6), 1969, pp. 1034-1041
Copyright © 1969 by The American Society of Tropical Medicine and Hygiene

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Begum, F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Begum, F.

Langat Virus

Differentiation of Strains of Reduced Pathogenicity Suitable for Vaccines*

Fatima Begum{dagger}
Pakistan Medical Research Center, University of Maryland, 6, Birdwood Road, Lahore, Pakistan

Two plaque-purified strains of Langat virus were studied to find markers that would distinguish strains of reduced pathogenicity suitable for vaccines. The following properties were examined: 1) pathogenicity in mice, 2) plaque morphology in chick-embryo cell cultures and the course of multiplication and antigen production, 3) thermal-inactivation rates, 4) chromatography on calcium phosphate columns, and 6) antigenic differences shown with the modified hemagglutination-inhibition (HI) test of Casals. The plaque assay, with the serum-free agar-overlay medium, was found to be as sensitive as the titration of the infectiousness of virus in mice, but plaque morphology was much the same for the two viruses. The two strains could, however, be differentiated on the basis of degree of peripheral pathogenicity in mice, thermal-inactivation rates, and difference in elution patterns and in antigenicity demonstrable by the modified HI test. The markers presently known still appear to be inadequate for the definition of Langat strains as suitable candidates for live vaccines against the tick-borne complex of group B arboviruses.


* This work, which was performed at the London School of Hygiene and Tropical Medicine, is a portion of the author's Ph.D. thesis, London University, 1963.


{dagger} Present address: c/o Dr. David F. Clyde, Institute of International Medicine, 660 West Redwood Street, Baltimore, Maryland 21201.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1969 by the American Society of Tropical Medicine and Hygiene.