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Am. J. Trop. Med. Hyg., 18(5), 1969, pp. 735-742
Copyright © 1969 by The American Society of Tropical Medicine and Hygiene

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Isolation of Viruses of the California Encephalitis Virus Group from Boreal Aedes Mosquitoes*

John Iversen{dagger}, R. P. Hanson, Orestes Papadopoulos{ddagger}, C. V. Morris AND G. R. DeFoliart
Departments of Veterinary Science and Entomology, The University of Wisconsin, Madison, Wisconsin 53706

During the summer of 1964 and the spring and summer of 1965, California encephalitis group viruses were isolated from six pools of boreal Aedes mosquitoes collected in and near Rochester (54°N, 113°W), Alberta, Canada. Mosquitoes were collected by aspiration, by sweep-netting, and with a CO2-baited cone trap. Collections were placed on Dry Ice and shipped to Madison, Wisconsin, for identification and isolation of virus. Aedes mosquitoes accounted for 97% of 16,453 mosquitoes examined for virus. Snowshoe hare virus of the California group was isolated from three pools of Aedes communis group, and one pool of Aedes stimulans group. Jamestown Canyon virus of the California group was isolated from one pool of A. communis and from a pool of Aedes spp. The minimum vector-infection rate was 0.40 isolates per 1,000 Aedes spp. mosquitoes in 1964, 0.46 isolates per 1,000 A. communis group mosquitoes in 1965, and 0.33 isolates per 1,000 A. stimulans group mosquitoes in 1965.


* Supported in part by grants AI 04725 and AI 00175 from the National Institutes of Health.


{dagger} Present address: Sonoma State College, Sonoma, California.


{ddagger} Present address: Department of Bacteriology and Parasitology, Faculty of Veterinary Medicine, Aristotelian University, Thessaloniki, Greece.






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