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Am. J. Trop. Med. Hyg., 17(5), 1968, pp. 695-701
Copyright © 1968 by The American Society of Tropical Medicine and Hygiene

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Antigens of Plasmodium Gallinaceum

II. Immunoserologic Characterization of Explasmodial Antigens and Their Antibodies*

Radmilo Todorovic, Deam H. Ferris AND Miodrag Ristic
Department of Veterinary Pathology and Hygiene, College of Veterinary Medicine, University of Illinois, Urbana 61801

Explasmodial antigens of Plasmodium gallinaceum were analyzed electrophoretically and found to consist of two fractions. Fluorescein-labeled antibody against explasmodial antigen was used to stain erythrocytes from chickens acutely infected with P. gallinaceum. In erythrocytes containing immature parasites, fluorescence was confined to the parasites only, whereas in erythrocytes containing mature schizonts, diffuse fluorescence of the entire erythrocyte was observed. The FA reaction was prevented by treatment of erythrocytes with nonconjugated anti-explasmodial globulin before treatment with conjugated globulin.

In vitro exposure of erythrocytes from chickens acutely infected with P. gallinaceum to hyperimmune explasmodial serum resulted in agglutination and lysis of infected erythrocytes, which resulted in liberation of the parasite. Inoculation of freed parasites in susceptible chickens resulted in P. gallinaceum infection.

Antibody to explasmodial antigen enhanced in vitro phagocytosis of normal chicken erythrocytes by mouse reticuloendothelial cells. Indirect evidence of similar in vivo activity of this antibody was also obtained.


* This investigation was supported in part by National Institutes of Health Research Grant AI-03315. The information presented is part of a thesis submitted by the senior author to the University of Illinois in partial fulfillment of the requirements for the Doctor of Philosophy degree.






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Copyright © 1968 by the American Society of Tropical Medicine and Hygiene.