| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
A method of fecal examination described by Kato and involving the clearing of a direct fecal smear pressed thin under a glycerin-impregnated cellophane coverslip was evaluated for possible use in quantitative diagnosis of helminth infections. Modifications were described for 1) removing fiber from the fecal sample, 2) uniformly spreading the smear, and 3) preventing over-clearing of the preparation. Optimum time for clearing the smears was found to be about 30 minutes at 35°C, or about 1 hour under openroom conditions.
The thick-smear method was found to be as sensitive as the Bell filtration, zine-surface flotation, and Hoffman sedimentation methods of concentration of feces. Egg-counts for estimation of intestinal nematode and Schistosoma mansoni infections were as reproducible when made by the thick-smear method as by dilution or concentration procedures.
Series of egg-counts made from the same stool and from daily stools indicated that eggs of S. mansoni, as well as those of other worms, are distributed randomly in the feces and are in equal numbers in the outer and inner portions of the stool. Thus egg-counts made by the thick-smear technique appear to be reliable for the quantitative diagnosis of helminth infect ons.
* This study was supported by grants AI-00002 and AI-04919 from the National Institutes of Health, U. S. Public Health Service.
This article has been cited by other articles:
|
|
 |
|
  P. Steinmann, Z.-W. Du, L.-B. Wang, X.-Z. Wang, J.-Y. Jiang, L.-H. Li, H. Marti, X.-N. Zhou, and J. Utzinger Extensive Multiparasitism in a Village of Yunnan Province, People's Republic of China, Revealed by a Suite of Diagnostic Methods Am J Trop Med Hyg, May 1, 2008; 78(5): 760 - 769. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Z. Long, J. L. Rosado, Y. Montoya, M. de Lourdes Solano, E. Hertzmark, H. L. DuPont, and J. I. Santos Effect of Vitamin A and Zinc Supplementation on Gastrointestinal Parasitic Infections Among Mexican Children Pediatrics, October 1, 2007; 120(4): e846 - e855. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Z. Long, J. I. Santos, T. Estrada Garcia, M. Haas, M. Firestone, J. Bhagwat, H. L. DuPont, E. Hertzmark, J. L. Rosado, and N. N. Nanthakumar Vitamin A Supplementation Reduces the Monocyte Chemoattractant Protein-1 Intestinal Immune Response of Mexican Children J. Nutr., October 1, 2006; 136(10): 2600 - 2605. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Raso, P. Vounatsou, B. H. Singer, E. K. N'Goran, M. Tanner, and J. Utzinger An integrated approach for risk profiling and spatial prediction of Schistosoma mansoni-hookworm coinfection PNAS, May 2, 2006; 103(18): 6934 - 6939. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Z. Long, T. Estrada-Garcia, J. L. Rosado, J. I. Santos, M. Haas, M. Firestone, J. Bhagwat, C. Young, H. L. DuPont, E. Hertzmark, et al. The Effect of Vitamin A Supplementation on the Intestinal Immune Response in Mexican Children Is Modified by Pathogen Infections and Diarrhea J. Nutr., May 1, 2006; 136(5): 1365 - 1370. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Raso, A. Luginbuhl, C. A Adjoua, N. T Tian-Bi, K. D Silue, B. Matthys, P. Vounatsou, Y. Wang, M.-E. Dumas, E. Holmes, et al. Multiple parasite infections and their relationship to self-reported morbidity in a community of rural Cote d'Ivoire Int. J. Epidemiol., October 1, 2004; 33(5): 1092 - 1102. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. El-Gohary, A.-E. Yassin, and M. A Shalabya The effect of chronic lead exposure on the course of schistosomiasis in hamsters and the protective effect of the antioxidant preparation 'Antox' Human and Experimental Toxicology, September 1, 2003; 22(9): 481 - 490. [Abstract] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
