AJTMH Transactions of the Royal Society of Tropical Medicine and Hygiene
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Am. J. Trop. Med. Hyg., 16(4), 1967, pp. 483-486
Copyright © 1967 by The American Society of Tropical Medicine and Hygiene

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An Improved Perfusion Technique for Recovering Adult Schistosomes from Laboratory Animals

Rodney H. Duvall AND William B. DeWitt
Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Public Health Service, U. S. Department of Health, Education, and Welfare, Bethesda, Maryland 20014

The technique described is the result of more than 15 years' experience in recovering adult schistosomes from experimentally infected animals. Basically, the method consists in administering intraperitoneally a combined injection of pentobarbital sodium (Nembutal) and heparin to produce a shift of the worms from the mesenteric veins to the liver and to prevent clotting while they are perfused directly into conical sedimentation glasses for collection and counting. The method permits the recovery of almost every live schistosome; it is rapid and simple.




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Copyright © 1967 by the American Society of Tropical Medicine and Hygiene.