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A total of 166 sera from adult residents in a northern Italian area (Gorizia province) was examined by the hemagglutination-inhibition test for antibodies against 10 arthropod-borne viruses: two from group A, five from group B, and Bunyamwera and Phlebotomus-fever (Sicilian and Neapolitan strains).
Fifty-eight sera (35%) reacted with one or more antigens. No evidence was found of immunity to viruses of group A or to Bunyamwera virus. Twenty sera (12%) reacted with group B viruses, two monotypically with TBE, eight monotypically with WN, and three with a titer with WN greater than with other group B antigens. One serum reacted at very high dilution (1:81, 820) with dengue 1; this donor had lived in Egypt until 1962.
Some of the HI-positive sera were further tested with TBE and WN viruses by the mouse-neutralization test. The presence of antibodies was confirmed in the two sera reacting monotypically with TBE. WN-neutralizing antibodies were not evidenced, except in one serum, indicating perhaps the presence of a closely related virus.
Forty-five sera (27%) reacted with Phlebotomus-fever antigens (Sicilian and Neapolitan strains) in the HI test. NT antibodies to the Sicilian strain were detected only in two sera among the HI-positive tested.
* Part of this work was done by one of us (P.V.) at the WHO Reference Laboratory for Arthropodborne viruses in the Institute of Virology, Czechoslovak Academy of Sciences, Bratislava, Czechoslovakia.
Department of Microbiology, Istituto Superiore di Sanità, Rome.
Public Health Officer, Gorizia.
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