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Am. J. Trop. Med. Hyg., 15(6), 1966, pp. 902-907
Copyright © 1966 by The American Society of Tropical Medicine and Hygiene

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Growth and Asexual Multiplication in Vitro of Mesocestoides Tetrathyridia*

Marietta Voge AND Lynne Shore Coulombe
Department of Medical Microbiology and Immunology, University of California, Los Angeles

A number of monophasic and diphasic media, with different gas phases and pH ranges, were used in the cultivation of Mesocestoides tetrathyridia in vitro. Growth and multiplication were evaluated by determining increases in the number of organisms and increases in protein after different periods of culture. In diphasic media containing whole human blood, asexual multiplication in vitro occurred consistently at a rate comparable to that in the mouse host. Good growth and some multiplication was obtained in medium containing human plasma.


* Supported in part by research grant No. AI 05835 from the National Institutes of Health, U. S. Public Health Service, Bethesda, Maryland.






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Copyright © 1966 by the American Society of Tropical Medicine and Hygiene.