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Isolation of Japanese Encephalitis Virus from the Blood of a Child in Singapore^*

The paper describes the clinical history and the isolation of a strain of Japanese encephalitis virus from the blood of a child. The virus was successfully re-isolated from the original acute phase serum and identified by complement-fixation, neutralization and hemagglutination inhibition antibody absorption tests. The etiological relationship of the virus to the patient's illness was established by the demonstration of complement-fixing, hemagglutination inhibition and neutralizing antibodies to the virus in the convalescent phase serum.

^* This work was supported by a grant to the Department of Bacteriology from the Rockefeller Foundation, New York.